IDR - IIT Kharagpur

Development of Liquid Culture and Machine Vision Systems for Efficient Micropropagation of Gladiolus Hybridus Hort

Development of Liquid Culture and Machine Vision Systems for Efficient Micropropagation of Gladiolus Hybridus Hort


Development Of Liquid Culture And Machine Vision Systems For Efficient Micropropagation Of Gladiolus Hybridus Hort: Multiple shoot clusters of gladiolus were established in semi-solid agar (AS) and liquid media appended with membrane raft (MR) and Duroplast foam (DF) support matrix. Maximum regeneration of 33.15 shoots/ cluster was obtained with 1 mg l-1 NAA and 2 mg l-1 BAP in liquid medium with membrane support. MS medium supplemented with 0.2 mg l-1 NAA and 2.0 mg l-1 BAP was found to be optimum with 21.34 shoots per cluster when solidified with agar, whereas 0.5 mg l-1 NAA and 4 mg l-1 BAP yielded a maximum of 24.74 shoots per cluster in DF system. There were 33.46 % and 25.37 % increase in shoot regeneration in MR system than AS and DF systems respectively. Shoot multiplication was rapid in AS and MR systems (relative differentiation rate of 5.7 % per 7d) when compared to DF system (relative differentiation rate of 4.6 % per 7d). Shoots grew to a maximum length of 1.37 cm in DF system whereas, in AS and MR systems, the shoots attained a length of 1.08 and 0.79 cm respectively at 35d. The average rate of shoot elongation (dY/dX ratio) in DF was 0.24, whereas in AS and MR systems the ratios were 0.16 and 0.12 respectively. Shoots in DF system exhibited faster elongation than those in AS and MR systems. Shoot multiplication followed a linear trend with incubation time, whereas shoot elongation fitted well with quadratic functions. Such analysis suggests that shoot number increases steadily with time, whereas the rate of shoot elongation was different at different phases of incubation in different culture systems. The kinetics of in vitro shoot multiplication was evaluated with Poisson regression analysis. The speed and acceleration derivatives of the fitted Poisson regression models suggested that significantly higher number of multiple axillary buds appeared with enhanced multiplication rate in MR culture compared to AS and DF. In MR and DF supported culture, the maximum speed of shoot multiplication was observed at 18d of incubation, whereas in AS system maximum speed was attained at 21d of incubation. The effect of Triadimefon (TDM) on regeneration of shoot clusters of gladiolus was also evaluated in both semi-solid (AS) and liquid (MR) culture systems. TDM at 1.0 mg l-1 concentration increased the number of shoots in both the culture types. The derivatives of the Poisson regression models suggested that TDM enhanced the speed of multiplication compared to controls irrespective of culture types.

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